ARG55404
anti-CD73 antibody [AD2]
anti-CD73 antibody [AD2] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections and Human,Primates
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [AD2] recognizes CD73 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr |
| Specificity | The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AD2 |
| Isotype | IgG1, kappa |
| Target Name | CD73 |
| Antigen Species | Human |
| Immunogen | Pre-B leukemia cells. |
| Conjugation | Un-conjugated |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Purification with Protein A. |
|---|---|
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NT5E |
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Highlight | Related products: CD73 antibodies; CD73 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 63 kDa |
Images (1) Click the Picture to Zoom In
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ARG55404 anti-CD73 antibody [AD2] CyTOF image
CyTOF: PBMC (after Ficoll-Paque separation) stained with ARG55404 anti-CD73 antibody [AD2] (Yb176).
