ARG57668
anti-CD75 / ST6GAL1 antibody
anti-CD75 / ST6GAL1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD75 / ST6GAL1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD75 / ST6GAL1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human CD75 / ST6GAL1. |
| Conjugation | Un-conjugated |
| Alternate Names | SIAT1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; B-cell antigen CD75; Sialyltransferase 1; ST6Gal I; Beta-galactoside alpha-2,6-sialyltransferase 1; EC 2.4.99.1; ST6N; Alpha 2,6-ST 1; ST6GalI |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse lung |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ST6GAL1 |
| Gene Full Name | ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 |
| Background | This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009] |
| Function | Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG57668 anti-CD75 / ST6GAL1 antibody WB image
Western blot: 25 µg of Mouse lung lysate stained with ARG57668 anti-CD75 / ST6GAL1 antibody at 1:1000 dilution.
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Review for anti-CD75 / ST6GAL1 antibody
Application：WB
Sample：Mouse hybridoma
Sample Loading Amount：50 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
