ARG58041
anti-CD86 antibody
anti-CD86 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD86 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD86 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human CD86. |
| Conjugation | Un-conjugated |
| Alternate Names | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | Raji | ||||||||||||
| Observed Size | ~ 66 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42081 Human T-lymphocyte activation antigen CD86 |
|---|---|
| Gene Symbol | CD86 |
| Gene Full Name | CD86 molecule |
| Background | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] |
| Cellular Localization | Cell membrane > Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 38 kDa |
| PTM | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG58041 anti-CD86 antibody ICC/IF image
Immunofluorescence: K562 cells stained with ARG58041 anti-CD86 antibody.
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ARG58041 anti-CD86 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG58041 anti-CD86 antibody.
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ARG58041 anti-CD86 antibody WB image
Western blot: Raji cell lysate stained with ARG58041 anti-CD86 antibody.
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ARG58041 anti-CD86 antibody FACS image
Flow Cytometry: K562 cells stained with ARG58041 anti-CD86 antibody at 1:50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti-Rabbit IgG was used as the secondary antibody.
