ARG22560
anti-CD88 / C5AR1 antibody [20/70] (PE)
anti-CD88 / C5AR1 antibody [20/70] (PE) for Flow cytometry and Mouse
Overview
| Product Description | PE-conjugated Rat Monoclonal antibody [20/70] recognizes CD88 / C5AR1 This antibody recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Clone 20/70 has been reported to block the binding of the C5a to murine CD88, (Soruri et al. 2003). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 20/70 |
| Isotype | IgG2b |
| Target Name | CD88 / C5AR1 |
| Antigen Species | Mouse |
| Immunogen | RBL-2H3 transfected cells expressing murine CD88 / C5aR. |
| Conjugation | PE |
| Alternate Names | CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30993 Mouse C5a anaphylatoxin chemotactic receptor 1 |
|---|---|
| Gene Symbol | C5ar1 |
| Gene Full Name | complement component 5a receptor 1 |
| Function | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. [UniProt] |
| Calculated MW | 39 kDa |
| PTM | Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042). Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338. |
