ARG62958
anti-CD99R antibody [MEM-131]
anti-CD99R antibody [MEM-131] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-131] recognizes CD99R |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, IP, WB |
| Specificity | The clone MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-131 |
| Isotype | IgM |
| Target Name | CD99R |
| Antigen Species | Human |
| Immunogen | HPB-ALL human peripheral blood leukemia T-cell line |
| Conjugation | Un-conjugated |
| Alternate Names | 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Thymic lymphocytes |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD99 |
| Gene Full Name | CD99 molecule |
| Background | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013] |
| Function | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). [UniProt] |
| Research Area | Cancer antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 19 kDa |
| PTM | Extensively O-glycosylated. |
