ARG66605
anti-CEP135 antibody
anti-CEP135 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CEP135 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CEP135 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1050-1130 of Human CEP135. |
| Conjugation | Un-conjugated |
| Alternate Names | Centrosomal protein of 135 kDa; KIAA0635; MCPH8; CEP4; Cep135; Centrosomal protein 4 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CEP135 |
| Gene Full Name | centrosomal protein 135kDa |
| Background | This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012] |
| Function | Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles. [UniProt] |
| Calculated MW | 133 kDa |
Images (2) Click the Picture to Zoom In
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ARG66605 anti-CEP135 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66605 anti-CEP135 antibody at 1:100 dilution (4°C, overnight). Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0). Negative control (right) was pre-absorbed by immunogen peptide.
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ARG66605 anti-CEP135 antibody WB image
Western blot: Mouse lung, Mouse heart and 293T cell lysates stained with ARG66605 anti-CEP135 antibody at 1:1000 dilution.
