ARG57499
anti-CHI3L2 antibody [2B5]
anti-CHI3L2 antibody [2B5] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2B5] recognizes CHI3L2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2B5 |
| Isotype | IgG2b, kappa |
| Target Name | CHI3L2 |
| Antigen Species | Human |
| Immunogen | Recombinant Human CHI3L2 (aa. 27-390) purified from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Chondrocyte protein 39; CHIL2; YKL-39; Chitinase-3-like protein 2; YKL39 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHI3L2 |
| Gene Full Name | chitinase 3-like 2 |
| Background | The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Function | Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity. [UniProt] |
| Calculated MW | 44 kDa |
Images (2) Click the Picture to Zoom In
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ARG57499 anti-CHI3L2 antibody [2B5] FACS image
Flow Cytometry: HeLa cells stained with ARG57499 anti-CHI3L2 antibody [2B5] at 2 - 5 µg/10^6 cells (red). Goat anti-Mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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ARG57499 anti-CHI3L2 antibody [2B5] WB image
Western blot: 40 µg of 293T, HeLa, HepG2 and LnCap cell lysates stained with ARG57499 anti-CHI3L2 antibody [2B5] at 1:1000 dilution.
