ARG20118
anti-CHOP / GADD153 antibody
anti-CHOP / GADD153 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHOP / GADD153 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Specificity | The antibody recognizes 25 kDa GADD153 of human, mouse, and rat origins. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHOP / GADD153 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide surrounding amino acid 124 of human GADD153 |
| Conjugation | Un-conjugated |
| Alternate Names | DNA damage-inducible transcript 3 protein; CHOP10; Growth arrest and DNA damage-inducible protein GADD153; GADD153; CCAAT/enhancer-binding protein homologous protein; C/EBP-homologous protein; CHOP; CHOP-10; C/EBP zeta; CEBPZ; DDIT-3; C/EBP-homologous protein 10 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified Antibody |
| Buffer | PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 30% Glycerol, 0.5% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35638 Human DNA damage-inducible transcript 3 protein Swiss-port # P35639 Mouse DNA damage-inducible transcript 3 protein |
|---|---|
| Background | GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells. |
| Research Area | Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 19 kDa |
| PTM | Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity. |
Specific References
Mangiferin alleviates endoplasmic reticulum stress in acute liver injury by regulating the miR-20a/miR-101a-Nrf2 axis.
WB / Mouse
