ARG45801

anti-CHP2 antibody

anti-CHP2 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CHP2
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CHP2
Antigen Species Human
Immunogen Recombinant protein containing to human CHP2.
Conjugation Un-conjugated
Alternate Names CHP2; Calcineurin Like EF-Hand Protein 2; Hepatocellular Carcinoma-Associated Antigen 520; Calcineurin B Homologous Protein 2; Hepatocellular Carcinoma Antigen Gene 520; HCA520

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 22 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 63928 Human CHP2

Swiss-port # O43745 Human Calcineurin B homologous protein 2

Gene Symbol CHP2
Gene Full Name Calcineurin Like EF-Hand Protein 2
Background This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013]
Function Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus. [UniProt]
Cellular Localization Cell membrane; Cytoplasm; Membrane; Nucleus. [UniProt]
Calculated MW 22 kDa
PTM Lipoprotein; Myristate; Phosphoprotein. [UniProt]