ARG57013
anti-CISD1 / mitoNEET antibody [1A8]
anti-CISD1 / mitoNEET antibody [1A8] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1A8] recognizes CISD1 / mitoNEET |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A8 |
| Isotype | IgG1, kappa |
| Target Name | CISD1 / mitoNEET |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 32-108 of Human CISD1 / mitoNEET. |
| Conjugation | Un-conjugated |
| Alternate Names | MDS029; mitoNEET; C10orf70; CDGSH iron-sulfur domain-containing protein 1; MitoNEET; ZCD1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZ45 Human CDGSH iron-sulfur domain-containing protein 1 |
|---|---|
| Gene Symbol | CISD1 |
| Gene Full Name | CDGSH iron sulfur domain 1 |
| Background | This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012] |
| Function | Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions. [UniProt] |
| Calculated MW | 12 kDa |
| PTM | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
Images (3) Click the Picture to Zoom In
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] FACS image
Flow Cytometry: THP-1 cell line stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] WB image
Western blot: 40 µg of Mouse liver lysate stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 1:1000.
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] WB image
Western blot: 40 µg of 1) K562 cell lysate, 2) MCF7 cell lysate stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 1:1000.
