ARG58438
anti-CKM / Creatine Kinase M antibody
anti-CKM / Creatine Kinase M antibody for Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes CKM / Creatine Kinase M |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CKM / Creatine Kinase M |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human CKM / Creatine phosphokinase M type (NP_001815.2). (C-QKIEEIFKKAGHP) |
| Conjugation | Un-conjugated |
| Alternate Names | CKMM; M-CK; Creatine kinase M chain; EC 2.7.3.2; Creatine kinase M-type |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CKM |
| Gene Full Name | creatine kinase, muscle |
| Background | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008] |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt] |
| Calculated MW | 43 kDa |
Images (3) Click the Picture to Zoom In
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ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35 µg of Human heart and Mouse heart lysates (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.03 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
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ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35 µg of Human (A) Pig (B) and Rat (C) heart lysates (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.3 µg/ml (A, B) and 0.1 µg/ml (C) dilutions and incubated at RT for 1 hour.
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ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35µg of Pig heart lysate (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.3 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
