ARG66114
anti-CTGF antibody
anti-CTGF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CTGF |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CTGF |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human CTGF. (GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA) |
| Conjugation | Un-conjugated |
| Alternate Names | Insulin-like growth factor-binding protein 8; IGFBP-8; CCN2; IBP-8; HCS24; Connective tissue growth factor; IGF-binding protein 8; CCN family member 2; IGFBP8; Hypertrophic chondrocyte-specific protein 24; NOV2 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTGF |
| Gene Full Name | connective tissue growth factor |
| Background | The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009] |
| Function | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. [UniProt] |
| Calculated MW | 38 kDa |
Images (4) Click the Picture to Zoom In
-
ARG66114 anti-CTGF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive malignant carcinoma. The recommended ARG66114 anti-CTGF antibody concentration is 0.125 µg/ml - 0.250 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
-
ARG66114 anti-CTGF antibody WB image
Western blot: 250 - 0.24 ng of Human CTGF stained with ARG66114 anti-CTGF antibody, under non-reducing conditions.
-
ARG66114 anti-CTGF antibody standard curve image
Sandwich ELISA: ARG66114 anti-CTGF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66115 anti-CTGF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
-
ARG66114 anti-CTGF antibody WB image
Western blot: 250 - 0.24 ng of Human CTGF stained with ARG66114 anti-CTGF antibody, under reducing conditions.
