ARG41572
anti-CYP19A1 / Aromatase antibody
anti-CYP19A1 / Aromatase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CYP19A1 / Aromatase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CYP19A1 / Aromatase |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to Y241-H503 of Human CYP19A1 / Aromatase. |
| Conjugation | Un-conjugated |
| Alternate Names | ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 58 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CYP19A1 |
| Gene Full Name | cytochrome P450, family 19, subfamily A, polypeptide 1 |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Function | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. [UniProt] |
| Cellular Localization | Membrane; Peripheral membrane protein. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41572 anti-CYP19A1 / Aromatase antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta lysate stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41572 anti-CYP19A1 / Aromatase antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal Goat serum and then stained with ARG41572 anti-CYP19A1 / Aromatase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
