ARG59195
anti-Cullin 5 antibody
anti-Cullin 5 antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cullin 5 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cullin 5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A2-D105 of Human Cullin 5. |
| Conjugation | Un-conjugated |
| Alternate Names | VACM-1; CUL-5; VACM1; Vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CUL5 |
| Gene Full Name | cullin 5 |
| Function | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. [UniProt] |
| Calculated MW | 91 kDa |
| PTM | Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59195 anti-Cullin 5 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat spleen, Rat heart, Rat kidney, Mouse brain, Mouse spleen, Mouse heart, Mouse kidney and Mouse testis lysates stained with ARG59195 anti-Cullin 5 antibody at 0.5 µg/ml, overnight at 4°C.
