ARG10876

anti-Cyclin B2 antibody [X29.2]

anti-Cyclin B2 antibody [X29.2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal,Xenopus laevis

Overview

Product Description Mouse Monoclonal antibody [X29.2] recognizes Cyclin B2
Tested Reactivity Hu, Ms, Rat, Mamm, Xenopus laevis
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity This antibody cross reacts with Cyclin B1.
Host Mouse
Clonality Monoclonal
Clone X29.2
Isotype IgG1
Target Name Cyclin B2
Antigen Species Xenopus laevis
Immunogen Xenopus laevis Cyclin B2.
Conjugation Un-conjugated
Alternate Names HsT17299; G2/mitotic-specific cyclin-B2

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Xenopus laevis testis.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12442 Mouse CCNB2

GeneID: 397743 Xenopus laevis CCNB2

GeneID: 9133 Human CCNB2

Gene Symbol CCNB2
Gene Full Name cyclin B2
Background Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt]
Calculated MW 45 kDa