ARG58464
anti-Cytochrome b5 antibody
anti-Cytochrome b5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytochrome b5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytochrome b5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-100 of Human Cytochrome b5 (NP_683725.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Microsomal cytochrome b5 type A; MCB5; Cytochrome b5; CYB5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HT29 | ||||||||
| Observed Size | 17 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CYB5A |
| Gene Full Name | cytochrome b5 type A (microsomal) |
| Background | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane, Single-pass membrane protein, Cytoplasmic side, Microsome membrane, Cytoplasm. [UniProt] |
| Calculated MW | 15 kDa |
Images (1) Click the Picture to Zoom In
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ARG58464 anti-Cytochrome b5 antibody WB image
Western blot: 25 µg of HT29 cell lysate stained with ARG58464 anti-Cytochrome b5 antibody at 1:1000 dilution.
