ARG57003
anti-Cytokeratin 23 antibody [2F6]
anti-Cytokeratin 23 antibody [2F6] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2F6] recognizes Cytokeratin 23 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2F6 |
| Isotype | IgG1, kappa |
| Target Name | Cytokeratin 23 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to aa. 271-422 of Human Cytokeratin 23. |
| Conjugation | Un-conjugated |
| Alternate Names | K23; CK23; CK-23; Keratin-23; HAIK1; Cytokeratin-23; Keratin, type I cytoskeletal 23 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT23 |
| Gene Full Name | keratin 23, type I |
| Background | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| Calculated MW | 48 kDa |
Images (3) Click the Picture to Zoom In
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ARG57003 anti-Cytokeratin 23 antibody [2F6] ICC/IF image
Immunoflorescense: Hep3B cells stained with ARG57003 anti-Cytokeratin 23 antibody [2F6] at 1:100 (green). DAPI (blue) for nucleus staining.
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ARG57003 anti-Cytokeratin 23 antibody [2F6] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57003 anti-Cytokeratin 23 antibody [2F6] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57003 anti-Cytokeratin 23 antibody [2F6] WB image
Western blot: 40 µg of HeLa and HepG2 cell lysates stained with ARG57003 anti-Cytokeratin 23 antibody [2F6] at 1:3000 dilution.
