ARG51680
anti-DARPP32 phospho (Thr75) antibody
anti-DARPP32 phospho (Thr75) antibody for ICC/IF and Human,Mouse,Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DARPP32 phospho (Thr75) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DARPP32 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from Human DARPP-32. |
| Conjugation | Un-conjugated |
| Alternate Names | Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPP1R1B |
| Gene Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 1B |
| Background | Inhibitor of protein-phosphatase 1. |
| Function | Inhibitor of protein-phosphatase 1. [UniProt] |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
| PTM | Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity. |
Images (1) Click the Picture to Zoom In
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ARG51680 anti-DARPP32 phospho (Thr75) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51680 anti-DARPP32 phospho (Thr75) antibody.
