ARG63459
anti-DCTN1 / p150-glued antibody
anti-DCTN1 / p150-glued antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DCTN1 / p150-glued |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DCTN1 / p150-glued |
| Antigen Species | Human |
| Immunogen | C-QEQLHQLHSRLIS |
| Conjugation | Un-conjugated |
| Alternate Names | P135; p150-glued; p135; DP-150; Dynactin subunit 1; 150 kDa dynein-associated polypeptide; DAP-150 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 142 kDa |
| PTM | Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase. |
Images (6) Click the Picture to Zoom In
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ARG63459 anti-DCTN1 / p150-glued antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63459 anti-DCTN1 / p150-glued antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63459 anti-DCTN1 / p150-glued antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum. (Steamed antigen retrieval with citrate buffer pH 6.0) stained with ARG63459 anti-DCTN1 / p150-glued antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63459 anti-DCTN1 / p150-glued antibody WB image
Western blot: Human testis lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63459 anti-DCTN1 / p150-glued antibody at 1 µg/ml dilution.
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ARG63459 anti-DCTN1 / p150-glued antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG63459 anti-DCTN1 / p150-glued antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63459 anti-DCTN1 / p150-glued antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63459 anti-DCTN1 / p150-glued antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63459 anti-DCTN1 / p150-glued antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63459 anti-DCTN1 / p150-glued antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
