ARG63168
anti-DDAH2 antibody
anti-DDAH2 antibody for Flow cytometry,ICC/IF,Western blot and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DDAH2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | FACS, ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDAH2 |
| Antigen Species | Human |
| Immunogen | SSLCLVLSTRPHS |
| Conjugation | Un-conjugated |
| Alternate Names | Dimethylarginine dimethylaminohydrolase 2; S-phase protein; G; DDAH; NG30; G6a; EC 3.5.3.18; N; Dimethylargininase-2; HEL-S-277; DDAH-2; Protein G6a; DDAHII |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95865 Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 |
|---|---|
| Background | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
| Research Area | Neuroscience antibody |
| Calculated MW | 30 kDa |
Images (3) Click the Picture to Zoom In
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ARG63168 anti-DDAH2 antibody WB image
Western Blot: Human Lung lysate (35 µg protein in RIPA buffer) stained with ARG63168 anti-DDAH2 antibody at 0.1 µg/ml dilution.
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ARG63168 anti-DDAH2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63168 anti-DDAH2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63168 anti-DDAH2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG63168 anti-DDAH2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
