ARG63911
anti-DDX6 antibody
anti-DDX6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DDX6 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDX6 |
| Antigen Species | Human |
| Immunogen | C-AEYHSEPVEDEKP |
| Conjugation | Un-conjugated |
| Alternate Names | Probable ATP-dependent RNA helicase DDX6; DEAD box protein 6; Oncogene RCK; ATP-dependent RNA helicase p54; EC 3.6.4.13; RCK; P54; HLR2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P26196 Human Probable ATP-dependent RNA helicase DDX6 |
|---|---|
| Gene Symbol | DDX6 |
| Gene Full Name | DEAD-box helicase 6 |
| Background | This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012] |
| Function | In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573). [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 54 kDa |
| PTM | Sumoylated (PubMed:26184334). |
Images (4) Click the Picture to Zoom In
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ARG63911 anti-DDX6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63911 anti-DDX6 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63911 anti-DDX6 antibody WB image
Western blot: 35 µg of Daudi (A), Jurkat (B) and HepG2 (C) lysates (in RIPA buffer) stained with ARG63911 anti-DDX6 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63911 anti-DDX6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63911 anti-DDX6 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63911 anti-DDX6 antibody WB image
Western blot: 1) Mock transfection and 2) Human DDX6 (RC209431) expressing plasmid transfected HEK293 cell lysate stained with ARG63911 anti-DDX6 antibody.
