ARG54370
anti-DEDAF antibody
anti-DEDAF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DEDAF |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Specificity | This antibody recognizes human, mouse, and rat DEDAF (32kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DEDAF |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 215-229 of human DEDAF (accession no. AF179286). |
| Conjugation | Un-conjugated |
| Alternate Names | YEAF1; RING1 and YY1-binding protein; DED-associated factor; Death effector domain-associated factor; YY1 and E4TF1-associated factor 1; AAP1; Apoptin-associating protein 1; DEDAF; APAP-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549 and HepG2 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RYBP |
| Gene Full Name | RING1 and YY1 binding protein |
| Background | Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. A novel protein that interacts with the death effector domain of capase-8 and caspase-10 has been designated DEDAF and is dedentical to the transcriptional repressor RYBP. DEDAF is expressed in multiple tissues and cell lines and appears to be involved in the regulation of both cytoplasmic and nuclear events of apoptosis. |
| Function | Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 25 kDa |
| PTM | Monoubiquitinated. |
Images (3) Click the Picture to Zoom In
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ARG54370 anti-DEDAF antibody WB image
Western Blot: A:A549; B:HepG2; C:mouse 3T3 stained with ARG54370 anti-DEDAF antibody at 1 μg/ml dilution.
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ARG54370 anti-DEDAF antibody IHC-P image
Immunohistochemistry: mouse liver stained with ARG54370 anti-DEDAF antibody at 20 μg/ml dilution.
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ARG54370 anti-DEDAF antibody IHC-P image
Immunohistochemistry: mouse liver stained with ARG54370 anti-DEDAF antibody at 5 μg/ml dilution.
