ARG58472
anti-DGKA antibody
anti-DGKA antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DGKA |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DGKA |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-200 of Human DGKA (NP_958852.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Diglyceride kinase alpha; DGK-alpha; 80 kDa diacylglycerol kinase; DAGK; DAGK1; DAG kinase alpha; EC 2.7.1.107; Diacylglycerol kinase alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | NCI-H460 | ||||
| Observed Size | 83 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DGKA |
| Gene Full Name | diacylglycerol kinase, alpha 80kDa |
| Background | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Function | Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. [UniProt] |
| Calculated MW | 83 kDa |
Images (1) Click the Picture to Zoom In
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ARG58472 anti-DGKA antibody WB image
Western blot: 25 µg of NCI-H460 cell lysate stained with ARG58472 anti-DGKA antibody at 1:1000 dilution.
