ARG43427
anti-DHODH antibody
anti-DHODH antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DHODH |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DHODH |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 132-173 of Human DHODH. (RVFRL PEDQA VINRY GFNSH GLSVV EHRLR ARQQK QAKLT ED) |
| Conjugation | Un-conjugated |
| Alternate Names | URA1; POADS; DHOdehase; Dihydroorotate dehydrogenase (quinone), mitochondrial; DHOdehase; EC 1.3.5.2; Dihydroorotate oxidase |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 43 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DHODH |
| Gene Full Name | dihydroorotate dehydrogenase (quinone) |
| Background | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein. [UniProt] |
| Calculated MW | 43 kDa |
| PTM | The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody WB image
Western blot: 30 µg of sample under reducing conditions. Rat liver, Mouse spleen and HepG2 whole cell lysates stained with ARG43427 anti-DHODH antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
