ARG20538

anti-DMPO Nitrone Adduct antibody [N166A4]

Datasheet

Ordering Information

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50 μg

335.00

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Datasheet

Overview

Product Description	Mouse Monoclonal antibody [N1664A] recognizes DMPO Nitrone Adduct
Tested Reactivity	Other
Tested Application	ELISA, ICC/IF, IHC-P, IP, WB
Specificity	Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Host	Mouse
Clonality	Monoclonal
Clone	N1664A
Isotype	IgG1
Target Name	DMPO Nitrone Adduct
Immunogen	5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
Conjugation	Un-conjugated

Application Instructions

Application Suggestion

Tested Application	Dilution
ELISA	1:100
ICC/IF	1:100
IHC-P	1:100
IP	25 µg/ml
WB	1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative	0.09% Sodium azide
Stabilizer	50% Glycerol
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background	The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
Research Area	Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Background

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.

Research Area

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Images (2) Click the Picture to Zoom In

ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] ICC/IF image

Immunofluorescence: Mouse macrophage cell lines stained with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] at 10 ug/ml dilution.
ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] WB image

Western blot: 1) 10 uM Hb (Hemoglobin), 2) 10 uM Hb + 100 uM HOCI, 3) 10 uM Hb + 500 uM HOCI, 4) 10 uM Hb + 1000 uM HOCI, 5)-8) as above with 20 mM DMPO, 9)-12) as above with 100 mM DMPO. Staining with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4].