ARG65650
anti-DMRT2 antibody
anti-DMRT2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DMRT2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognize isoform 2 only (NP_870987.2). Reported variants NP_001124337.1 and NP_006548.1 represent identical protein |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | DMRT2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human DMRT2 (C-KRPSSAITRVSQ) |
| Conjugation | Un-conjugated |
| Alternate Names | Doublesex-like 2 protein; Doublesex- and mab-3-related transcription factor 2; DSXL-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y5R5 Human Doublesex- and mab-3-related transcription factor 2 |
|---|---|
| Gene Symbol | DMRT2 |
| Gene Full Name | doublesex and mab-3 related transcription factor 2 |
| Background | The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. [provided by RefSeq, Apr 2010] |
| Function | Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome (By similarity). [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 62 kDa |
Images (1) Click the Picture to Zoom In
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ARG65650 anti-DMRT2 antibody IHC image
Immunohistochemistry: paraffin embedded Human Kidney stained with ARG65650 anti-DMRT2 antibody at 2 μg/ml dilution. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
