ARG22625
anti-Dityrosine antibody [10A6]
anti-Dityrosine antibody [10A6] for ICC/IF,Western blot and Other
Overview
| Product Description | Mouse Monoclonal antibody [10A6] recognizes Dityrosine |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, WB |
| Specificity | Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10A6 |
| Isotype | IgG1 |
| Target Name | Dityrosine |
| Antigen Species | Others |
| Immunogen | Synthetic Dityrosine conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| Preservative | 0.9% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
-
ARG22625 anti-Dityrosine antibody [10A6] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22625 anti-Dityrosine antibody [10A6] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite.
-
ARG22625 anti-Dityrosine antibody [10A6] WB image
Western blot: 1 µg of BSA, 3,5-Dibromotyrosine-BSA, Dityrosine-BSA, Bromotyrosine-BSA and 7-ketocholesterol-BSA stained with ARG22625 anti-Dityrosine antibody [10A6] at 1:1000 for 2 hours at RT.
-
ARG22625 anti-Dityrosine antibody [10A6] WB image
Western blot: 12 µg of HeLa cell lysate and H2O2 treated HeLa cell lysate stained with ARG22625 anti-Dityrosine antibody [10A6] at 1:1000 for 2 hours at RT.
