ARG65435
anti-Drebrin antibody [DBN-N-03]
anti-Drebrin antibody [DBN-N-03] for CyTOF®-candidate,Flow cytometry,Western blot and Human
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [DBN-N-03] recognizes Drebrin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, WB |
| Specificity | The clone DBN-N-03 recognizes drebrin, an approximately 100-125 kDa regulator of actin cytoskeleton. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DBN-N-03 |
| Isotype | IgG2b |
| Target Name | Drebrin |
| Antigen Species | Human |
| Immunogen | Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326) |
| Conjugation | Un-conjugated |
| Alternate Names | Developmentally-regulated brain protein; D0S117E; Drebrin |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | WB: REH, Nalm-6 and HEK293. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DBN1 |
| Gene Full Name | drebrin 1 |
| Background | Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse. |
| Function | Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. [UniProt] |
| Research Area | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 71 kDa |
Images (2) Click the Picture to Zoom In
-
ARG65435 anti-Drebrin antibody [DBN-N-03] WB image
Western blot: 1. REH cell lysate 2. Ramos cell lysate 3. Nalm-6 cell lysate stained with ARG65435 anti-Drebrin antibody [DBN-N-03].
-
ARG65435 anti-Drebrin antibody [DBN-N-03] CyTOF image
CyTOF: Human brain tumor cells were stained with ARG65435 anti-Drebrin antibody [DBN-N-03] (152Sm). Singlet cells were gated for data analysis.
