Overview

Product Description	Rabbit Polyclonal antibody recognizes E2F2
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	E2F2
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human E2F2.
Conjugation	Un-conjugated
Alternate Names	Transcription factor E2F2; E2F-2

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:100 WB 1:1000 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	K562
Observed Size	~ 50 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1870 Human E2F2 GeneID: 242705 Mouse E2F2 Swiss-port # P56931 Mouse Transcription factor E2F2 Swiss-port # Q14209 Human Transcription factor E2F2
Gene Symbol	E2F2
Gene Full Name	E2F transcription factor 2
Background	The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]
Function	Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	48 kDa
PTM	Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. [UniProt]

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