ARG63995
anti-EBAG9 / RCAS1 antibody
anti-EBAG9 / RCAS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes EBAG9 / RCAS1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EBAG9 / RCAS1 |
| Antigen Species | Human |
| Immunogen | C-KEAQRLMKKEQN |
| Conjugation | Un-conjugated |
| Alternate Names | Cancer-associated surface antigen RCAS1; Receptor-binding cancer antigen expressed on SiSo cells; Estrogen receptor-binding fragment-associated gene 9 protein; EB9; PDAF |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00559 Human Receptor-binding cancer antigen expressed on SiSo cells |
|---|---|
| Background | This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
Images (2) Click the Picture to Zoom In
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ARG63995 anti-EBAG9 / RCAS1 antibody WB image
Western Blot: A431 lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. stained with ARG63995 anti-EBAG9 / RCAS1 antibody at 0.2 µg/ml dilution.
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ARG63995 anti-EBAG9 / RCAS1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63995 anti-EBAG9 / RCAS1 antibody at 3.75 µg/ml dilution followed by AP-staining.
