ARG58653
anti-EHHADH antibody
anti-EHHADH antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EHHADH |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EHHADH |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 444-723 of Human EHHADH (NP_001957.2). |
| Conjugation | Un-conjugated |
| Alternate Names | LBP; ECHD; LBFP; L-PBE; PBE; PBFE; EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8; FRTS3; Peroxisomal bifunctional enzyme |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | SW480 | ||||
| Observed Size | 79 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EHHADH |
| Gene Full Name | enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase |
| Background | The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Cellular Localization | Peroxisome. [UniProt] |
| Calculated MW | 79 kDa |
| PTM | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG58653 anti-EHHADH antibody WB image
Western blot: 25 µg of SW480 cell lysate stained with ARG58653 anti-EHHADH antibody at 1:1000 dilution.
