ARG59414
anti-EME1 antibody
anti-EME1 antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EME1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EME1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-320 of Human EME1 (NP_689676.2). |
| Conjugation | Un-conjugated |
| Alternate Names | MMS4 homolog; MMS4L; SLX2A; EC 3.1.22.-; Crossover junction endonuclease EME1; hMMS4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Rat thymus and Mouse thymus | ||||
| Observed Size | 63 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8BJW7 Mouse Crossover junction endonuclease EME1 |
|---|---|
| Gene Symbol | EME1 |
| Gene Full Name | essential meiotic structure-specific endonuclease 1 |
| Background | This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009] |
| Function | Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. [UniProt] |
| Cellular Localization | Nucleus, nucleolus. Note=Recruited to regions of DNA damage in S-phase cells. [UniProt] |
| Calculated MW | 63 kDa |
Images (1) Click the Picture to Zoom In
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ARG59414 anti-EME1 antibody WB image
Western blot: 25 µg of Rat thymus and Mouse thymus lysates stained with ARG59414 anti-EME1 antibody at 1:1000 dilution.
