ARG22476

anti-EMR1 F4 / 80 antibody [Cl:A3-1]

Datasheet

Publication6

Ordering Information

Package

Price (USD)

100 μg

389.00

Add to cart

Overview

Product Description	Rat Monoclonal antibody [Cl:A3-1] recognizes EMR1 F4 / 80
Tested Reactivity	Ms, Rat
Tested Application	EM, FACS, ICC/IF, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB
Specificity	EMR1 F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family.
Host	Rat
Clonality	Monoclonal
Clone	Cl:A3-1
Isotype	IgG2b
Target Name	EMR1 F4 / 80
Antigen Species	Mouse
Immunogen	Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Conjugation	Un-conjugated
Alternate Names	EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3

Application Instructions

Application Suggestion

Tested Application	Dilution
EM	5 - 10 µg/ml
FACS	1:50 - 1:100 (Use 10 µl to label 10^6 cells in 100 µl)
ICC/IF	5 - 10 µg/ml
IHC-Fr	5 - 10 µg/ml
IHC-P	5 - 10 µg/ml
IHC-R	Assay-dependent
IP	Assay-dependent
RIA	Assay-dependent
WB	1:500 - 1:1000

Application Note

IHC-P: Antigen Retrieval: Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS and 0.09% Sodium azide.
Preservative	0.09% Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 13733 Mouse ADGRE1 GeneID: 316137 Rat ADGRE1 Swiss-port # Q5Y4N8 Rat Adhesion G protein-coupled receptor E2 Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1
Gene Symbol	Adgre1
Gene Full Name	adhesion G protein-coupled receptor E1
Background	EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function	EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]
Highlight	Related Antibody Duos and Panels: ARG30326 Inflammatory Cell Antibody Panel (for mouse) Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response
Research Area	Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
Calculated MW	98 kDa

Images (4) Click the Picture to Zoom In

ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] ICC/IF image

Immunofluorescence: Rat tumor macrophages stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1].
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

Immunohistochemistry: Mouse spleen cryosection stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-Fr image

Immunohistochemistry: Frozen section of Mouse liver tissue stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image

Immunohistochemistry: A. Mouse spleen stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution. B. Primary antibodies preincubated with 2 molar excess of Human anti Idiotypic.

Specific References

Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

IHC-P / Mouse

Hao Yu et al.
Front Microbiol., (2023)

DNA-Based Hydrogels with Multidrug Sequential Release for Promoting Diabetic Wound Regeneration

IHC-P / Mouse

Wei Li et al.
JACS Au., (2023)

Voglibose Regulates the Secretion of GLP-1 Accompanied by Amelioration of Ileal Inflammatory Damage and Endoplasmic Reticulum Stress in Diabetic KKAy Mice

IHC-P / Mouse

Yaxin Fu et al.
Int J Mol Sci, (2022)

Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer

ARG22476: IHC-P / Mouse

Li-Hsien Wu et al.
J Cancer, (2022)

Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.

IHC-P / Mouse

Quan Liu et al.
Front Pharmacol., (2021)

Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency.

IHC-P / Mouse

Xuelian Tang et al.
J Biomed Sci., (2021)