ARG22200
anti-ENaC alpha antibody
anti-ENaC alpha antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat,Xenopus laevis
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ENaC alpha |
|---|---|
| Tested Reactivity | Ms, Rat, Xenopus laevis |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~85kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ENaC alpha |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around aa. 46-68 (N-temrinus) of Rat ENaC Alpha |
| Conjugation | Un-conjugated |
| Alternate Names | ENaCalpha; ENaCa; Epithelial Na; Amiloride-sensitive sodium channel subunit alpha; ENaCA; SCNEA; Alpha-ENaC; BESC2; Nonvoltage-gated sodium channel 1 subunit alpha; Alpha-NaCH; SCNN1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Scnn1a |
| Gene Full Name | sodium channel, non-voltage-gated 1 alpha subunit |
| Background | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009] |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. [UniProt] |
| Cellular Localization | Apical cell membrane |
| Calculated MW | 76 kDa |
| PTM | Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation. N-glycosylated. |
Images (3) Click the Picture to Zoom In
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ARG22200 anti-ENaC alpha antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat kidney tissue stained with ARG22200 anti-ENaC alpha antibody (green) at 1:25 dilution for 1 hour at RT. Phalloidin-Alexa Fluor 633 (red) for actin staining. DAPI (blue) for nuclear staining.
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ARG22200 anti-ENaC alpha antibody WB image
Western blot: Rat kidney tissue lysates stained with ARG22200 anti-ENaC alpha antibody at 1:1000 dilution.
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ARG22200 anti-ENaC alpha antibody WB image
Western blot: Mouse kidney tissue lysates stained with ARG22200 anti-ENaC alpha antibody.
