ARG10729
anti-Enolase 1 antibody [253]
anti-Enolase 1 antibody [253] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [253] recognizes Enolase 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 253 |
| Isotype | IgG1 |
| Target Name | Enolase 1 |
| Antigen Species | Bovine |
| Immunogen | N-terminal 12 aa. of Bovine Enolase1. |
| Conjugation | Un-conjugated |
| Alternate Names | MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO1 |
| Gene Full Name | enolase 1, (alpha) |
| Background | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| Function | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | ISGylated. |
Images (4) Click the Picture to Zoom In
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ARG10729 anti-Enolase 1 antibody [253] WB image
Western blot: 20 µg of U2OS and HeLa cell lysates stained with ARG10729 anti-Enolase 1 antibody [253] at 1:5000 dilution.
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ARG10729 anti-Enolase 1 antibody [253] ICC/IF image
Immunocytochemistry: NIH/3T3 cells stained with ARG10729 anti-Enolase 1 antibody [253] (green) and co-stained with chicken polyclonal antibody to vimentin (red); DNA (blue). ARG10729 reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments.
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ARG10729 anti-Enolase 1 antibody [253] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10729 anti-Enolase 1 antibody [253] (green) at 1:500 dilution and costained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.
Clone 253 reveals strong cytoplasmic staining while the Hsp 60 antibody specifically labels mitochondria in these cells.
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ARG10729 anti-Enolase 1 antibody [253] WB image
Western blot: NIH/3T3, C6, HEK293, HeLa and SH-SY5Y cell lysates stained with ARG10729 anti-Enolase 1 antibody [253] (green) at 1:10000 dilution.
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Review for anti-Enolase 1 antibody [253]
Application：WB
Sample：Rat heart
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
