ARG45959
anti-FACT / SUPT16H antibody
anti-FACT / SUPT16H antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FACT / SUPT16H |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FACT / SUPT16H |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human FACT / SUPT16H. |
| Conjugation | Un-conjugated |
| Alternate Names | SUPT16H; SPT16 Homolog, Facilitates Chromatin Remodeling Subunit; Chromatin-specific transcription elongation factor 140 kDa subunit; FACTP140; FACT complex subunit SPT16; SPT16; FACTp140; CDC68; FACT 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; SPT16/CDC68; hSPT16 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 140 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SUPT16H |
| Gene Full Name | SPT16 Homolog, Facilitates Chromatin Remodeling Subunit |
| Background | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009] |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). [UniProt] |
| Cellular Localization | Nucleus; Chromosome. [UniProt] |
| Calculated MW | 120 kDa |
| PTM | Phosphoprotein; Isopeptide bond; Ubl conjugation; Acetylation; ADP-ribosylation. [UniProt]. [UniProt] |
