ARG65617
anti-FADD antibody
anti-FADD antibody for Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FADD. |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FADD |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human FADD (NP_003815.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Mediator of receptor induced toxicity; MORT1; GIG3; FAS-associated death domain protein; Growth-inhibiting gene 3 protein; Protein FADD; FAS-associating death domain-containing protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | ~ 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13158 Human FAS-associated death domain protein Swiss-port # Q61160 Mouse FAS-associated death domain protein |
|---|---|
| Gene Symbol | FADD |
| Gene Full Name | Fas (TNFRSF6)-associated via death domain |
| Background | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008] |
| Function | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt] |
| Highlight | Related products: FADD antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 23 kDa |
Images (2) Click the Picture to Zoom In
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ARG65617 anti-FADD antibody WB image
Western blot: HeLa cell lysate stained with ARG65617 anti-FADD antibody.
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ARG65617 anti-FADD antibody IP image
Immunoprecipitation: 200 µg extracts of HeLa cells were immunoprecipitated and stained with ARG65617 anti-FADD antibody at 1:1000 dilution.
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Review for anti-FADD antibody
Application：WB
Sample：293T and HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
