ARG41851
anti-FBXO32 antibody [M314]
anti-FBXO32 antibody [M314] for Western blot and Human
Overview
| Product Description | Rat Monoclonal antibody [M314] recognizes FBXO32 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | M314 |
| Isotype | IgG |
| Target Name | FBXO32 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide (coupled to carrier protein) corresponding to aa. 23-35 of Mouse FBXO32. |
| Conjugation | Un-conjugated |
| Alternate Names | Muscle atrophy F-box protein; Atrogin-1; F-box only protein 32; Fbx32; MAFbx |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FBXO32 |
| Gene Full Name | F-box protein 32 |
| Background | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and the nucleus. [UniProt] |
| Calculated MW | 42 kDa |
Images (1) Click the Picture to Zoom In
-
ARG41851 anti-FBXO32 antibody [M314] WB image
Western blot: Human FBXO32 recombinant protein stained with ARG41851 anti-FBXO32 antibody [M314] at 1:1000, 1:2000 and 1:4000 dilution.
