ARG58002

anti-FBXO34 antibody

anti-FBXO34 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Hamster

Overview

Product Description Rabbit Polyclonal antibody recognizes FBXO34
Tested Reactivity Hu, Hm
Predict Reactivity Ms
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FBXO34
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 600-629 (C-terminus) of Human FBXO34.
Conjugation Un-conjugated
Alternate Names Fbx34; CGI-301; F-box only protein 34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control CHO
Observed Size ~ 78 kDa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55030 Human FBXO34

Swiss-port # Q9NWN3 Human F-box only protein 34

Gene Symbol FBXO34
Gene Full Name F-box protein 34
Background Members of the F-box protein family, such as FBXO34, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. [UniProt]
Calculated MW 79 kDa

Images (3) Click the Picture to Zoom In

  • ARG58002 anti-FBXO34 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma tissue stained with ARG58002 anti-FBXO34 antibody.

  • ARG58002 anti-FBXO34 antibody WB image

    Western blot: 35 µg of CHO cell lysate stained with ARG58002 anti-FBXO34 antibody.

  • ARG58002 anti-FBXO34 antibody FACS image

    Flow Cytometry: WiDr cells stained with ARG58002 anti-FBXO34 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC-labelled secondary antibody.