ARG53014
anti-FBXW7 antibody [SP237]
anti-FBXW7 antibody [SP237] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP237] recognizes FBXW7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP237 |
| Isotype | IgG |
| Target Name | FBXW7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from the internal region of human FBXW7 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | AGO; F-box protein FBX30; hCdc4; FBW7; FBW6; F-box/WD repeat-containing protein 7; SEL-10; hAgo; FBX30; SEL10; FBXO30; F-box and WD-40 domain-containing protein 7; CDC4; FBXW6; Archipelago homolog |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Endometrial Adenocarcinoma, Lung Squamous Cell Carcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q969H0 Human F-box/WD repeat-containing protein 7 |
|---|---|
| Background | F-box/WD repeat-containing protein 7 (Fbxw7) is part of an ubiquitin-protein ligase complex. The complex ubiquitinates and degrades several important oncogenes such as mTOR, MYC, AURKA, JUN, and NOTCH1. Fbxw7 cooperates with PTEN for tumor suppression and is widely expressed in many normal tissues. The loss of fbxw7 is associated with malignant progression in a variety of tumors, including carcinoma of colorectum, lung, stomach, bile duct, endometrium, pancreas, breast and glioblastoma. |
| Cellular Localization | Cytoplasm, Nucleus, Nucleolus |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody |
| Calculated MW | 80 kDa |
| PTM | Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923). Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its degradation (PubMed:22608923). |
Images (1) Click the Picture to Zoom In
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ARG53014 anti-FBXW7 antibody [SP237] IHC-P image
Immunohistochemistry: Human Endometrial Adenocarcinoma stained with FBXW7 antibody [SP237] (ARG53014)
