ARG64070
anti-FEZ1 / Zygin 1 antibody
anti-FEZ1 / Zygin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FEZ1 / Zygin 1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform 1 (NP_005094.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FEZ1 / Zygin 1 |
| Antigen Species | Human |
| Immunogen | C-QNKQKEQRELMKK |
| Conjugation | Un-conjugated |
| Alternate Names | Fasciculation and elongation protein zeta-1; Zygin-1; Zygin I |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99689 Human Fasciculation and elongation protein zeta-1 |
|---|---|
| Background | This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| Calculated MW | 45 kDa |
| PTM | Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination. Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26. |
Images (3) Click the Picture to Zoom In
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ARG64070 anti-FEZ1 / Zygin 1 antibody WB image
Western Blot: Human Brain (frontal Cortex) lysate (35 µg protein in RIPA buffer) stained with ARG64070 anti-FEZ1 / Zygin 1 antibody at 0.03 µg/ml dilution.
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ARG64070 anti-FEZ1 / Zygin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64070 anti-FEZ1 / Zygin 1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64070 anti-FEZ1 / Zygin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64070 anti-FEZ1 / Zygin 1 antibody at 5 µg/ml dilution followed by AP-staining.
