ARG63657
anti-FHL1 / SLIM1 antibody
anti-FHL1 / SLIM1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes FHL1 / SLIM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FHL1 / SLIM1 |
| Antigen Species | Human |
| Immunogen | CNKRFVFHNEQVY |
| Conjugation | Un-conjugated |
| Alternate Names | FHL1A; FHL1B; KYOT; Four and a half LIM domains protein 1; SLIM; FLH1A; XMPMA; FHL-1; SLIM-1; SLIM1; SLIMMER; Skeletal muscle LIM-protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 36 kDa |
Images (2) Click the Picture to Zoom In
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ARG63657 anti-FHL1 / SLIM1 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG63657 anti-FHL1 / SLIM1 antibody at 10 µg/ml dilution.
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ARG63657 anti-FHL1 / SLIM1 antibody WB image
Western Blot: Human Muscle lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63657 anti-FHL1 / SLIM1 antibody at 0.05 µg/ml dilution.
