ARG44240
anti-FOXA2 / HNF3B antibody
anti-FOXA2 / HNF3B antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FOXA2 / HNF3B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FOXA2 / HNF3B |
| Antigen Species | Human |
| Immunogen | Human FOXA2 / HNF3B recombinant protein (a.a: 139-446) |
| Conjugation | Un-conjugated |
| Alternate Names | TCF3B; Hepatocyte nuclear factor 3-beta; Forkhead box protein A2; TCF-3B; HNF-3B; HNF3B; Transcription factor 3B; HNF-3-beta |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. |
Images (6) Click the Picture to Zoom In
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ARG44240 anti-FOXA2 / HNF3B antibody IHC-P image
Immunohistochemistry: Human colonic adenocarcinoma stained with ARG44240 anti-FOXA2 / HNF3B antibody at 2 μg / ml dilution.
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ARG44240 anti-FOXA2 / HNF3B antibody WB image
Western blot: HepG2, PC-3 and A549 stained with ARG44240 anti-FOXA2 / HNF3B antibody at 0.5 μg / ml dilution.
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ARG44240 anti-FOXA2 / HNF3B antibody ICC/IF image
Immunofluorescence: PC-3 stained with ARG44240 anti-FOXA2 / HNF3B antibody at 5 μg / ml dilution.
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ARG44240 anti-FOXA2 / HNF3B antibody FACS image
Flow Cytometry: 293T stained with ARG44240 anti-FOXA2 / HNF3B antibody at 1 μg / 10^6 cells dilution.
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ARG44240 anti-FOXA2 / HNF3B antibody WB image
Western blot: Rat pancreas stained with ARG44240 anti-FOXA2 / HNF3B antibody at 0.5 μg / ml dilution.
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ARG44240 anti-FOXA2 / HNF3B antibody WB image
Western blot: Mouse pancreas stained with ARG44240 anti-FOXA2 / HNF3B antibody at 0.5 μg / ml dilution.
