ARG62433
anti-FZR1 / CDH1 antibody [DH01 (DCS-266)]
anti-FZR1 / CDH1 antibody [DH01 (DCS-266)] for Flow cytometry,Immunoprecipitation,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [DH01 (DCS-266)] recognizes FZR1 / CDH1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DH01 (DCS-266) |
| Isotype | IgG1 |
| Target Name | FZR1 / CDH1 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein (Human) |
| Conjugation | Un-conjugated |
| Alternate Names | CDC20-like protein 1; hCDH1; FZR2; FZR; Fizzy-related protein homolog; HCDH1; HCDH; Fzr; CDH1; CDC20C; Cdh1/Hct1 homolog |
Application Instructions
| Application Note | IP: 2 µg/ml WB: 2 µg/ml FACS: 1: 10 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FZR1 |
| Gene Full Name | fizzy/cell division cycle 20 related 1 |
| Function | Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 55 kDa |
| PTM | Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159; deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK. |
Clone References
WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis.
6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27.
IP / Human
A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells.
WB / Human
Uncovering the role of APC-Cdh1 in generating the dynamics of S-phase onset.
Checkpoint kinase 1 is essential for meiotic cell cycle regulation in mouse oocytes.
WB / Mouse
Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
WB, IHC-P / Mouse
