ARG62486
anti-Factor XIIIa antibody [AC-1A1]
anti-Factor XIIIa antibody [AC-1A1] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Controls and Markers antibody
Overview
| Product Description | Mouse Monoclonal antibody [AC-1A1] recognizes Factor XIIIa |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This antibody is specific to 160 kDa protein known as Factor XIIIA. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AC-1A1 |
| Isotype | IgG1, kappa |
| Target Name | Factor XIIIa |
| Antigen Species | Human |
| Immunogen | Recombinant human protein corresponding to A-subunit of coagulation Factor XIII |
| Conjugation | Un-conjugated |
| Alternate Names | Coagulation factor XIIIa; F13A; Protein-glutamine gamma-glutamyltransferase A chain; Coagulation factor XIII A chain; Transglutaminase A chain; EC 2.3.2.13 |
Application Instructions
| Application Note | IHC-Fr: 1/25 - 1/50 FACS: 1µg for 106 cells * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | IgG purified |
| Buffer | PBS, 1% BSA and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F13A1 |
| Gene Full Name | coagulation factor XIII, A1 polypeptide |
| Background | This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008] |
| Function | Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. [UniProt] |
| Cellular Localization | Cytoplasm. Secreted |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody |
| Calculated MW | 83 kDa |
| PTM | The activation peptide is released by thrombin. |
Clone References
Regulation of gene expression by PI3K in mouse growth plate chondrocytes.
WB, IHC-P / Mouse
Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion.
WB, ICC/IF / Mouse
Plaque-like CD34-positive dermal fibroma (medallion-like dermal dendrocyte hamartoma): clinicopathologic, immunohistochemical, and molecular analysis of 5 cases emphasizing its distinction from superficial, plaque-like dermatofibrosarcoma protuberans.
IHC / Human
Fibrofolliculoma/trichodiscoma and fibrous papule (perifollicular fibroma/angiofibroma): a revaluation of the histopathological and immunohistochemical features.
IHC-P / Human
Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation.
ICC/IF / Human
Expression and localization of plasma transglutaminase factor XIIIA in bone.
WB / Human
