ARG63473
anti-Flotillin 1 antibody
anti-Flotillin 1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Flotillin 1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Flotillin 1 |
| Antigen Species | Human |
| Immunogen | C-SISQVNHKPLRTA |
| Conjugation | Un-conjugated |
| Alternate Names | Flotillin-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (3) Click the Picture to Zoom In
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ARG63473 anti-Flotillin 1 antibody WB image
Western Blot: H460 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63473 anti-Flotillin 1 antibody at 0.3 µg/ml dilution.
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ARG63473 anti-Flotillin 1 antibody WB image
Western Blot: 1). Mock transfection; 2) FLOT1(RC200231) expressing plasmid transfected HEK293 cell lysate standed with ARG63473 anti-Flotillin 1 antibody
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ARG63473 anti-Flotillin 1 antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG63473 anti-Flotillin 1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
