ARG52284
anti-Fractin antibody
anti-Fractin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Fractin |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Fractin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the C terminal region of the 32-kDa actin fragment |
| Conjugation | Un-conjugated |
| Alternate Names | CFTDM; MPFD; CFTD; ASMA; NEM1; NEM2; NEM3; Alpha-actin-1; ACTA; CFTD1; Actin, alpha skeletal muscle |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~ 32 kDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo prepaRations due to further degradation by other proteases. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Neat Serum |
| Buffer | Neat serum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | fragment of Actin |
| Gene Full Name | actin, alpha 1, skeletal muscle |
| Background | Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009) |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 42 kDa |
| PTM | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
Images (1) Click the Picture to Zoom In
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ARG52284 anti-Fractin antibody WB image
Western blot: Colchicin treated SY5Y cell lysate showing specific immunolabeling of the ~ 32 kDa cleaved actin fragment (fractin) stained with ARG52284 anti-Fractin antibody.
