ARG56602
anti-G-CSF antibody [500-M37]
anti-G-CSF antibody [500-M37] for ELISA,Neutralizing,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [500-M37] recognizes G-CSF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, Neut, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 500-M37 |
| Target Name | G-CSF |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human G-CSF. (TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP) |
| Conjugation | Un-conjugated |
| Alternate Names | Granulocyte colony-stimulating factor; Lenograstim; C17orf33; GCSF; G-CSF; Filgrastim; Pluripoietin; CSF3OS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09919 Human Granulocyte colony-stimulating factor |
|---|---|
| Gene Symbol | CSF3 |
| Gene Full Name | colony stimulating factor 3 (granulocyte) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). |
Images (3) Click the Picture to Zoom In
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ARG56602 anti-G-CSF antibody [500-M37] WB image
Western blot: 250 - 0.24 ng (left to right) of Human G-CSF stained with ARG56602 anti-G-CSF antibody [500-M37], under reducing conditions.
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ARG56602 anti-G-CSF antibody [500-M37] WB image
Western blot: 250 - 0.24 ng (left to right) of Human G-CSF stained with ARG56602 anti-G-CSF antibody [500-M37], under non-reducing conditions.
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ARG56602 anti-G-CSF antibody [500-M37] standard curve image
Sandwich ELISA: ARG56602 anti-G-CSF antibody [500-M37] as a capture antibody at 2.0 - 4.0 µg/ml combined with ARG56788 anti-G-CSF antibody (Biotin) as a detection antibody at ~ 0.5 - 1.0 µg/ml. Results of a typical standard run with optical density.
