ARG55132

anti-GATA3 antibody [1259CT707.136.102]

anti-GATA3 antibody [1259CT707.136.102] for Flow cytometry,ICC/IF,Western blot and Human

Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody recognizes GATA3
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 1259CT707.136.102
Isotype IgG2b, kappa
Target Name GATA3
Antigen Species Human
Immunogen Recombinant protein of Human GATA3.
Conjugation Un-conjugated
Alternate Names Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; HDR; HDRS

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25
ICC/IF1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HL-60

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2625 Human GATA3

Swiss-port # P23771 Human Trans-acting T-cell-specific transcription factor GATA-3

Gene Symbol GATA3
Gene Full Name GATA binding protein 3
Background This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. [UniProt]
Cellular Localization Nucleus.
Research Area Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody
Calculated MW 48 kDa

Images (3) Click the Picture to Zoom In

  • ARG55132 anti-GATA3 antibody ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG55132 anti-GATA3 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

  • ARG55132 anti-GATA3 antibody WB image

    Western blot: 35 µg of HL-60 cell lysate stained with ARG55132 anti-GATA3 antibody at 1:1000 dilution.

  • ARG55132 anti-GATA3 antibody FACS image

    Flow Cytometry: MCF7 cells stained with ARG55132 anti-GATA3 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.