ARG64150
anti-GJB2 / Connexin 26 antibody
anti-GJB2 / Connexin 26 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GJB2 / Connexin 26 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | FACS, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GJB2 / Connexin 26 |
| Antigen Species | Human |
| Immunogen | YLLIRYCSGKSKKP |
| Conjugation | Un-conjugated |
| Alternate Names | DFNB1; DFNA3; DFNA3A; PPK; Cx26; CX26; DFNB1A; HID; Gap junction beta-2 protein; NSRD1; Connexin-26; KID |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 26 kDa |
Images (3) Click the Picture to Zoom In
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ARG64150 anti-GJB2 / Connexin 26 antibody WB image
Western blot: 35 µg of Human colon (A), heart (B), prostate (C), skin (D) and adipose (E, negative control) lysates (in RIPA buffer) stained with ARG64150 anti-GJB2 / Connexin 26 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64150 anti-GJB2 / Connexin 26 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG64150 anti-GJB2 / Connexin 26 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64150 anti-GJB2 / Connexin 26 antibody WB image
Western blot: 35 µg of Mouse brain (A), Mouse heart (B) and Rat heart (C) lysates (in RIPA buffer) stained with ARG64150 anti-GJB2 / Connexin 26 antibody at 0.01 µg/ml (A) and 0.5 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
